![]() , Although described more than a century ago, the Gram stain remains the most frequently used rapid diagnostic test, and in conjunction with various biochemical tests is the cornerstone of the clinical laboratory. Available from: Įffective utilization and understanding of the clinical bacteriology laboratory can greatly aid in the diagnosis of infectious diseases. Laboratory perspective of gram staining and its significance in investigations of infectious diseases. How to cite this URL: Thairu Y, Nasir IA, Usman Y. How to cite this article: Thairu Y, Nasir IA, Usman Y. Keywords: Positive culture, Bacteriological, Limitations and special samples The aim of this mini review is to analyze and discuss the clinical significance and limitations of reporting Gram staining results for sample meant for bacteriological investigations. This would carry a risk leading to inadequate antimicrobial therapy and potentially affecting patients' clinical course and mortality. On the other hand, there remains the possibility that Gram staining results do not match with the final identification of microorganisms. In recent reports, the impact of Gram staining results on patient mortality has been documented. ![]() It is therefore highly likely that the information provided by the Gram staining will help to assess the adequacy of preliminary diagnosis and antimicrobial therapy selected after collecting culture specimens and before final identification of the microorganism. The most important and primary test to perform directly on some special samples such as cerebrospinal fluid and positive cultures is Gram staining which serves as the most rapid and simplest test to characterize microorganisms. Isolation, identification of pathogenic microorganisms in cultures and subsequent antimicrobial susceptibility testing always assists in selecting appropriate antimicrobial agent and prevention of unnecessary complications. Clinical microbiology laboratory plays several important roles in the management of bacterial infections. ![]()
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